The Mg2+ transporter CNNM4 regulates sperm Ca2+ homeostasis and is essential for reproduction.

The Mg 2 + transporter CNNM 4 regulates sperm Ca 2 + homeostasis and it is essential for reproduction

Basolateral Mg2+ Extrusion via CNNM4 Mediates Transcellular Mg2+ Transport across Epithelia: A Mouse Model

Transcellular Mg(2+) transport across epithelia, involving both apical entry and basolateral extrusion, is essential for magnesium homeostasis, but molecules involved in basolateral extrusion have not yet been identified. Here, we show that CNNM4 is the basolaterally located Mg(2+) extrusion molecule. CNNM4 is strongly expressed in intestinal epithelia and localizes to their basolateral membran...

Matrix free Mg2+ and the regulation of mitochondrial volume.

Mitochondria must maintain volume homeostasis in order to carry out oxidative phosphorylation. It has been postulated that the concentration of free Mg2+([Mg2+]) serves as the sensor of matrix volume and regulates a K+-extruding K+/H+antiport (K. D. Garlid. J. Biol. Chem. 255: 11273-11279, 1980). To test this hypothesis, the fluorescent probe furaptra was used to monitor [Mg2+] and free Ca2+ co...

Rebuttal from Francisco J. Arjona and Jeroen H. F. de Baaij

We fully agree with Funato and colleagues on the relevance of CNNM proteins for the maintenance of Mg2+ homeostasis (Funato et al. 2018). Both of our papers summarize the solid body of evidence demonstrating thatCNNM2 is key for renalMg2+ handling, while CNNM4 regulates intestinal Mg2+ transport. Nevertheless, we do not share the conclusions presented by Funato and colleagues suggesting that CN...

Metal-Ion-Coordinating Properties of Various Amino Acids, Investigation of the Essential Function in Biological Systems regarding to their Nano-Structure

The acidity constants of some amino acids (Am) were determined by potentiometric pH titration. The stability constants of the 1:1 complexes formed between M2+: Ca2+, Mg2+, Mn2+, Co2+, Ni2+, Cu2+ or Zn2+ and Am2-, were determined by potentiometric pH titration in aqueous solution (I = 0.1 M, NaNO3, 25°C). The order of the stability constants was reported. It is shown that the stability of the bi...